Changelog

Main user-facing changes are listed here. For a full list of changes, see the GitHub release notes of the frontend and backend repositories.

Using digestion fragments or oligonucleotid hybridization products in Golden Gate assembly

OpenCloning now supports using digestion fragments or oligonucleotid hybridization products in Golden Gate assembly. Previously, you could only provide sequences that would be restricted by the assembly enzymes as inputs. Now, you can provide an already restricted fragment as input. This is particularly useful if you use oligonucleotid hybridization products as inputs for custom DNA in Golden Gate assembly.

Snapgene history parsing

OpenCloning now parses the history of SnapGene files! You can just drag-and-drop a single .dna file into OpenCloning, and it will parse its history. You can see it in action in this LinkedIn post. If you want to use this in scripts, check out this function.

Phage recombinases

OpenCloning now supports custom phage recombinases. You can simulate site-specific recombination defining your own recognition sites (e.g. custom attB/attP/attL/attR sequences). Check out the documentation.

Assembler

The OpenCloning Assembler is a tool that allows you to clone with MoClo and Golden Gate kits. Designed to demistify Golden Gate assembly for newbies and to save time for experts, it makes combinatorial assembly using MoClo kits blazing fast. The best way to get a feeling of what it can do is to try it with one of the Syntaxes that are already available. Check out this demo video or the documentation.

Manual annotation

👉 Check out the documentation.

You can now manually annotate sequences (create features) and create primers directly in the editor. You can also delete and modify existing annotation.

Simplified python scripting for easier automation

You can now write python scripts to automate cloning directly using the python library pydna, which is integrated with the OpenCloning data model.

👉 Check out the documentation for how to get started with automating your cloning design!

Import sequences from Open DNA Collections

Open DNA Collections are distributed by the Reclone project. You can now import sequences from these collections directly into your design.